TOKSISITAS KOMBINASI EKSTRAK ROSELA (Hibiscus sabdariffa L.) DAN PARE (Momordica charantia) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

TOXICITY OF THE COMBINATION OF ROSELLA (Hibiscus sabdariffa L.) AND PARE (Momordica charantia) EXTRACTS USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

Abstract

Rosela (Hibiscus sabdariffa L.) dan pare (Momordica charantia) mengandung senyawa kimia yang bermanfaat bagi kesehatan, seperti asam fenolat, tannin, flavonoid, alkaloid, saponin, tanin, glikosida, dan steroid. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) terhadap larva Artemia salina Leach menggunakan metode Brine Shrimp Lethality Test (BSLT). Tingkat toksisitas tersebut ditunjukkan dengan nilai LC50. Metode: Penelitian uji toksisitas kombinasi ekstrak Hibiscus sabdariffa L dan Momordica charantia terhadap larva udang (Artemia salina L.) menggunakan metode BSLT. Dalam penelitian ini, ekstrak kombinasi bunga rosela dan buah pare diuji toksisitasnya terhadap larva Artemia salina Leach dengan konsentrasi 1000 ppm, 500 ppm, 200 ppm, 100 ppm, dan 50 ppm. Pengujian dilakukan sebanyak tiga kali pada masing-masing konsentrasi. Data hasil pengujian dianalisis menggunakan analisa probit, dan log konsentrasinya dihitung untuk mengetahui nilai LC50. Hasil : Ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) memiliki nilai LC50 sebesar 241,8 ppm.

Kata kunci: BSLT, Hibiscus sabdariffa L, Momordica charantia

Roselle (Hibiscus sabdariffa L.) and bitter melon (Momordica charantia) contain chemical compounds that are beneficial for health, such as phenolic acids, tannins, flavonoids, alkaloids, saponins, tannins, glycosides, and steroids. This study aims to determine the level of toxicity of the combined extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The LC50 value indicates the level of toxicity. Process: Research the toxicity test of a combination of Hibiscus sabdariffa L. and Momordica charantia extracts against shrimp larvae (Artemia salina L.) using the BSLT method. This research tested the combined extract of roselle flowers and bitter melon fruit for toxicity against Artemia salina Leach larvae at concentrations of 1000 ppm, 500 ppm, 200 ppm, 100 ppm, and 50 ppm. The test was carried out three times at each concentration. The test result data was analyzed using probit analysis, and the concentration log was calculated to determine the LC50 value. Results: The combination extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) had an LC50 value of 241.8 ppm.

Keywords: BSLT, Hibiscus sabdariffa L, Momordica charantia